Nrf2 [p Ser40] Antibody (SR2277)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21580

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21580-100ul
NBP3-21580-50ul
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2277

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nrf2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-Nrf2 [Ser40] (Uniprot #: Q16236)

Modification

p Ser 40

Specificity

Detects endogenous levels of total phospho-Nrf2 [Ser40]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nrf2 [p Ser40] Antibody (SR2277)

Immunohistochemistry: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] -

Immunohistochemistry: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NBP3-21580.

Western Blot: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] -

Western Blot: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Western blot analysis using NBP3-21580 in HepG2 cell lysate.

Immunocytochemistry/Immunofluorescence: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] -

Immunocytochemistry/Immunofluorescence: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Immunofluorescent analysis of HepG2 cells, using NBP3-21580.

Applications for Nrf2 [p Ser40] Antibody (SR2277)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nrf2

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Gene Symbol

NFE2L2

Additional Nrf2 Products

Product Documents for Nrf2 [p Ser40] Antibody (SR2277)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nrf2 [p Ser40] Antibody (SR2277)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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