NRXN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00219

Novus Biologicals, part of Bio-Techne
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NBP1-00219
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NRXN1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KEKQPSSAKSSNKN corresponding to C-Terminus according to NP_620072.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_004792.1 and NP_620072.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NRXN1 Antibody

Western Blot: NRXN1 Antibody [NBP1-00219]

Western Blot: NRXN1 Antibody [NBP1-00219]

Western Blot: NRXN1 Antibody [NBP1-00219] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with (1/3000) in lane C. Mock-transfected HEK293 probed with NBP1-00219 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Flow Cytometry: NRXN1 Antibody [NBP1-00219]

Flow Cytometry: NRXN1 Antibody [NBP1-00219]

Flow Cytometry: NRXN1 Antibody [NBP1-00219] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for NRXN1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 1 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60 kDa band were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NRXN1

Neurexins function in the vertebrate nervous system as cell adhesion molecules and receptors. Two neurexin genes are among the largest known in human (NRXN1 and NRXN3). By using alternate promoters, splice sites and exons, predictions of hundreds or even thousands of distinct mRNAs have been made. Most transcripts use the upstream promoter and encode alpha-neurexin isoforms; fewer transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. Alpha-neurexins contain epidermal growth factor-like (EGF-like) sequences and laminin G domains, and they interact with neurexophilins. Beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. The RefSeq Project has decided to create only a few representative transcript variants of the multitude that are possible. [provided by RefSeq]

Alternate Names

DKFZp313P2036, FLJ35941, KIAA0578Neurexin I-alpha, neurexin 1, neurexin I, Neurexin I-beta, neurexin-1-alpha, neurexin-1-beta, PTHSL2

Entrez Gene IDs

9378 (Human); 18189 (Mouse); 60391 (Rat)

Gene Symbol

NRXN1

UniProt

Q9ULB1 (Human)

Additional NRXN1 Products

Product Documents for NRXN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NRXN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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