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Nse2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76263

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MMS21. The immunogen is located within amino acids 170 - 220 of MMS21. Amino Acid Squence: CIVRMIESRQKRKKKA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nse2 Antibody - BSA Free

Western Blot: Nse2 AntibodyBSA Free [NBP1-76263]

Western Blot: Nse2 AntibodyBSA Free [NBP1-76263]

Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - Nse2 Antibody [NBP1-76263] - CRISPR-Cas9-mediated targeting of NSMCE2. Western blot analysis of the NSMCE2 protein in the final NSMCE2 null and WT cells. beta-Actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/17/11/1782) licensed under a CC-BY license.
Western Blot: Nse2 AntibodyBSA Free [NBP1-76263]

Western Blot: Nse2 AntibodyBSA Free [NBP1-76263]

Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - Nse2 Antibody [NBP1-76263] - 293 cell lysate with Nse2 antibody at (A) 0.5 and (B) 1 ug/mL.
Nse2 Antibody - BSA Free

Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] -

Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - CRISPR-Cas9-mediated targeting of NSMCE2: (A) Left panel, transfected U2OS cells (GFP+) under bright field & fluorescence; right panel, FACS enrichment of GFP+ cells. Bar = 50 µm; (B) Expected cleavage bands (approximately 304 & 113 bp, arrowheads) generated by Surveyor nuclease digestion. For negative control (−) transfection of the pX458 plasmids without NSMCE2 sgRNA was performed; (C) Sequencing analysis for characterization of the CRISPR-Cas9-induced frameshift mutations. Red letters represent the 20-nt targeting sequences, while blue letters refer to the protospacer-adjacent motif (PAM); (D) Western blot analysis of the NSMCE2 protein in the final NSMCE2 null & WT cells. beta-Actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/17/11/1782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Nse2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

0.5 - 1 ug/ml.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nse2

MMS21, also known as NSE2, is a SUMO ligase that in combination with the SMC5/6 complex is required for the prevention of DNA damage induced apoptosis by facilitating DNA repair in human cells. MMS21-dependent sumoylation is integral and important to the cohesion mechanism and mitotic progression; this function appears to be independent of SMC6. MMS21 mediates SUMO attachment to various proteins such as SMC6L1 and TRAX, and possibly the cohesin components RAD21 and STAG2.

Alternate Names

C8orf36, E3 SUMO-protein ligase NSE2, EC 6.3.2, EC 6.3.2.-, FLJ32440, hMMS21, methyl methanesulfonate sensitivity gene 21, MMS21 homolog, MMS21chromosome 8 open reading frame 36, Non-SMC element 2 homolog, non-SMC element 2 homolog (MMS21, S. cerevisiae), non-SMC element 2, MMS21 homolog (S. cerevisiae), Non-structural maintenance of chromosomes element 2 homolog, NSE2

Gene Symbol

NSMCE2

Additional Nse2 Products

Product Documents for Nse2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nse2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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