Nse2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76263
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MMS21. The immunogen is located within amino acids 170 - 220 of MMS21. Amino Acid Squence: CIVRMIESRQKRKKKA
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nse2 Antibody - BSA Free
Western Blot: Nse2 AntibodyBSA Free [NBP1-76263]
Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - Nse2 Antibody [NBP1-76263] - CRISPR-Cas9-mediated targeting of NSMCE2. Western blot analysis of the NSMCE2 protein in the final NSMCE2 null and WT cells. beta-Actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/17/11/1782) licensed under a CC-BY license.Western Blot: Nse2 AntibodyBSA Free [NBP1-76263]
Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - Nse2 Antibody [NBP1-76263] - 293 cell lysate with Nse2 antibody at (A) 0.5 and (B) 1 ug/mL.Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] -
Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - CRISPR-Cas9-mediated targeting of NSMCE2: (A) Left panel, transfected U2OS cells (GFP+) under bright field & fluorescence; right panel, FACS enrichment of GFP+ cells. Bar = 50 µm; (B) Expected cleavage bands (approximately 304 & 113 bp, arrowheads) generated by Surveyor nuclease digestion. For negative control (−) transfection of the pX458 plasmids without NSMCE2 sgRNA was performed; (C) Sequencing analysis for characterization of the CRISPR-Cas9-induced frameshift mutations. Red letters represent the 20-nt targeting sequences, while blue letters refer to the protospacer-adjacent motif (PAM); (D) Western blot analysis of the NSMCE2 protein in the final NSMCE2 null & WT cells. beta-Actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/17/11/1782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Nse2 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
0.5 - 1 ug/ml.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nse2
Alternate Names
C8orf36, E3 SUMO-protein ligase NSE2, EC 6.3.2, EC 6.3.2.-, FLJ32440, hMMS21, methyl methanesulfonate sensitivity gene 21, MMS21 homolog, MMS21chromosome 8 open reading frame 36, Non-SMC element 2 homolog, non-SMC element 2 homolog (MMS21, S. cerevisiae), non-SMC element 2, MMS21 homolog (S. cerevisiae), Non-structural maintenance of chromosomes element 2 homolog, NSE2
Gene Symbol
NSMCE2
UniProt
Additional Nse2 Products
Product Documents for Nse2 Antibody - BSA Free
Product Specific Notices for Nse2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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