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NSFL1C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13676

Novus Biologicals, part of Bio-Techne
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NBP2-13676
NBP2-13676-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDADEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NSFL1C Antibody

Western Blot: NSFL1C Antibody [NBP2-13676]

Western Blot: NSFL1C Antibody [NBP2-13676]

Western Blot: NSFL1C Antibody [NBP2-13676] - Analysis using Anti-NSFL1C antibody NBP2-13676 (A) shows similar pattern to independent antibody NBP2-13677 (B).
Immunocytochemistry/ Immunofluorescence: NSFL1C Antibody [NBP2-13676]

Immunocytochemistry/ Immunofluorescence: NSFL1C Antibody [NBP2-13676]

Immunocytochemistry/Immunofluorescence: NSFL1C Antibody [NBP2-13676] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13676]

Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13676]

Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13676] - Staining of human kidney.

Applications for NSFL1C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NSFL1C

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

dJ776F14.1, NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, P47, p97 cofactor p47, SHP1 homolog, UBX domain protein 2C, UBX domain-containing protein 2C, UBX1, UBXD10, UBXN2CMGC3347

Gene Symbol

NSFL1C

Additional NSFL1C Products

Product Documents for NSFL1C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NSFL1C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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