Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NSFL1C Antibodies »

Product Summary for NSFL1C Antibody

Immunogen

The immunogen recognized by the antibody maps to a region between residue 25 to 75 of human NSFL1 (p97) Cofactor (p47) using the numbering given in entry NP_057227.2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NSFL1C Antibody

Western Blot: NSFL1C Antibody [NBP2-36529]

Western Blot: NSFL1C Antibody [NBP2-36529]

Western Blot: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: NSFL1C Antibody [NBP2-36529]

Immunoprecipitation: NSFL1C Antibody [NBP2-36529]

Immunoprecipitation: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated NSFL1C, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for NSFL1C Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NSFL1C

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

dJ776F14.1, NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, P47, p97 cofactor p47, SHP1 homolog, UBX domain protein 2C, UBX domain-containing protein 2C, UBX1, UBXD10, UBXN2CMGC3347

Gene Symbol

NSFL1C

UniProt

NP_057227.2 (Human)

Additional NSFL1C Products

Product Documents for NSFL1C Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NSFL1C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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