NSUN4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19594
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human NSUN4. The exact sequence is proprietary.
Reactivity Notes
Bovine (83%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NSUN4 Antibody
Western Blot: NSUN4 Antibody [NBP2-19594]
Western Blot: NSUN4 Antibody [NBP2-19594] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibodyImmunohistochemistry-Paraffin: NSUN4 Antibody [NBP2-19594]
Immunohistochemistry-Paraffin: NSUN4 Antibody [NBP2-19594] - Human colon carcinoma, using NSUN4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Western Blot: NSUN4 Antibody [NBP2-19594]
Western Blot: NSUN4 Antibody [NBP2-19594] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.Applications for NSUN4 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NSUN4
Alternate Names
EC 2.1.1, EC 2.1.1.-, EC 2.1.1.29, MGC22960, NOL1/NOP2/Sun domain family 4, NOL1/NOP2/Sun domain family member 4, NOL1/NOP2/Sun domain family, member 4, NOP2/Sun domain family, member 4, putative methyltransferase NSUN4, RP4-603I14.2
Gene Symbol
NSUN4
UniProt
Additional NSUN4 Products
Product Documents for NSUN4 Antibody
Product Specific Notices for NSUN4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.