NTAL Antibody (NAP-07) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-341
![Immunocytochemistry/ Immunofluorescence: NTAL Antibody (NAP-07) - BSA Free [NB500-341]](https://resources.bio-techne.com/images/products/NTAL-Antibody-NAP-07-Immunocytochemistry-Immunofluorescence-NB500-341-img0001.jpg "Immunocytochemistry/ Immunofluorescence: NTAL Antibody (NAP-07) - BSA Free [NB500-341]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # NAP-07
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for NTAL Antibody (NAP-07) - BSA Free
Immunogen
Recombinant cytoplasmic domain (amino acids 91-244) of Human NTAL.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
The antibody NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NTAL Antibody (NAP-07) - BSA Free
Immunocytochemistry/ Immunofluorescence: NTAL Antibody (NAP-07) - BSA Free [NB500-341]
Immunocytochemistry/Immunofluorescence: NTAL Antibody (NAP-07) [NB500-341] - Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).Applications for NTAL Antibody (NAP-07) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
1-4 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:50
Western Blot
2 ug/ml
Application Notes
Western Blot - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NTAL
Long Name
Non-T Cell Activation Linker
Alternate Names
LAB, WBSCR15, WBSCR5
Gene Symbol
LAT2
Additional NTAL Products
Product Documents for NTAL Antibody (NAP-07) - BSA Free
Product Specific Notices for NTAL Antibody (NAP-07) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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