NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73082

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NUB1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-326 of human NUB1 (NP_057202) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free

Western Blot: NUB1/NYREN18 Antibody (OTI2G1)Azide and BSA Free [NBP2-73082]

Western Blot: NUB1/NYREN18 Antibody (OTI2G1)Azide and BSA Free [NBP2-73082]

Western Blot: NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free [NBP2-73082] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUB1.
Flow Cytometry: NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free [NBP2-73082]

Flow Cytometry: NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free [NBP2-73082]

Flow Cytometry: NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free [NBP2-73082] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by NUB1 antibody.
Western Blot: NUB1/NYREN18 Antibody (OTI2G1)Azide and BSA Free [NBP2-73082]

Western Blot: NUB1/NYREN18 Antibody (OTI2G1)Azide and BSA Free [NBP2-73082]

Western Blot: NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free [NBP2-73082] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications for NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NUB1

NUB1 interacts with and negatively regulates NEDD8 (MIM 603171), a ubiquitin-like protein that covalently conjugates to cullin (see MIM 603134) family members.[supplied by OMIM]

Long Name

NEDD8 Ultimate Buster 1

Alternate Names

BS4, NUB1L, NY-REN-18

Gene Symbol

NUB1

Additional NUB1 Products

Product Documents for NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NUB1/NYREN18 Antibody (OTI2G1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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