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NUCKS1 Antibody (3G10)

Novus Biologicals, part of Bio-Techne | Catalog # H00064710-M02

Novus Biologicals, part of Bio-Techne
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H00064710-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3G10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NUCKS1 (NP_073568.2, 1 a.a. ~ 243 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRPVRNRKVVDYSQFQESDDADEDYGRDSGPPTKKIRSSPREAKNKRRSGKNSQEDSEDSEDKDVKTKKDDSHSAEDSEDEKEDHKNVRQQRQAASKAASKQREMLMEDVGSEEEQEEEDEAPFQEKDSGSDEDFLMEDDDDSDYGSSKKKNKKMVKKSKPERKEKKMPKPRLKATVTPSPVKGKGKVGRPTASKASKEKTPSPKEEDEEPESPPEKKTSTSPPPEKSGDEGSEDEAPSGED

Specificity

This product is specific for Human NUCKS1 monoclonal antibody (M02), clone 3G10 [Gene ID: 64710].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NUCKS1 Antibody (3G10)

Immunocytochemistry/ Immunofluorescence: NUCKS1 Antibody (3G10) [H00064710-M02]

Immunocytochemistry/ Immunofluorescence: NUCKS1 Antibody (3G10) [H00064710-M02]

Immunocytochemistry/Immunofluorescence: NUCKS1 Antibody (3G10) [H00064710-M02] - Analysis of monoclonal antibody to NUCKS1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: NUCKS1 Antibody (3G10) [H00064710-M02] - Detection limit for recombinant GST tagged NUCKS1 is 0.3 ng/ml as a capture antibody.

Applications for NUCKS1 Antibody (3G10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml
Application Notes
Mouse monoclonal antibody raised against a full-length recombinant NUCKS1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NUCKS1

NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene. Alternate names for NUCKS include NUCKS1, JC7, and FLJ21480.

Alternate Names

FLJ21480, FLJ32016, FLJ38536, NUCKSnuclear ubiquitous casein and cyclin-dependent kinases substrate, nuclear casein kinase and cyclin-dependent kinase substrate 1, nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate, P1, potential LAG1 interactor

Gene Symbol

NUCKS1

Additional NUCKS1 Products

Product Documents for NUCKS1 Antibody (3G10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NUCKS1 Antibody (3G10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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