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NUCKS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49365

Novus Biologicals, part of Bio-Techne
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NBP2-49365
NBP2-49365-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for NUCKS1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MEDDDDSDYGSSKKKNKKMVKKSKPERKEKKMPKPRLKATVTPSPVKGKG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NUCKS1 Antibody

Immunocytochemistry/ Immunofluorescence: NUCKS1 Antibody [NBP2-49365]

Immunocytochemistry/ Immunofluorescence: NUCKS1 Antibody [NBP2-49365]

Immunocytochemistry/Immunofluorescence: NUCKS1 Antibody [NBP2-49365] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: NUCKS1 Antibody [NBP2-49365]

Immunohistochemistry-Paraffin: NUCKS1 Antibody [NBP2-49365]

Immunohistochemistry-Paraffin: NUCKS1 Antibody [NBP2-49365] - Staining of human prostate shows strong nuclear positivity in glandular cells.

Applications for NUCKS1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NUCKS1

NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene. Alternate names for NUCKS include NUCKS1, JC7, and FLJ21480.

Alternate Names

FLJ21480, FLJ32016, FLJ38536, NUCKSnuclear ubiquitous casein and cyclin-dependent kinases substrate, nuclear casein kinase and cyclin-dependent kinase substrate 1, nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate, P1, potential LAG1 interactor

Gene Symbol

NUCKS1

Additional NUCKS1 Products

Product Documents for NUCKS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NUCKS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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