Nuclear Factor Erythroid Derived 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31540
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (90%), Porcine (91%), Rat (90%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
Applications
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Nuclear Factor Erythroid Derived 2. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nuclear Factor Erythroid Derived 2 Antibody
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-31540-img0007.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540]")
Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540]
Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NF-E2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31540-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540]")
Immunocytochemistry/ Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540]
Immunocytochemistry/Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-E2 protein stained by NF-E2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.Applications for Nuclear Factor Erythroid Derived 2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Application Notes
ChIP-seq, IP Assay dependent
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nuclear Factor Erythroid Derived 2
Alternate Names
Leucine zipper protein NF-E2, NF-E2, nuclear factor (erythroid-derived 2), 45kD, nuclear factor (erythroid-derived 2), 45kDa, Nuclear factor, erythroid-derived 2 45 kDa subunit, p45, p45 NF-E2, transcription factor NF-E2 45 kDa subunit
Gene Symbol
NFE2
Additional Nuclear Factor Erythroid Derived 2 Products
Product Documents for Nuclear Factor Erythroid Derived 2 Antibody
Product Specific Notices for Nuclear Factor Erythroid Derived 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.