Nucleolin Antibody (NCL/7014R) [Janelia Fluor® 549]

Validated:

Janelia Fluor 549

Recombinant Monoclonal Rabbit IgG Kappa Clone # NCL/7014R

Please see the vial label for concentration. If unlisted please contact technical services.

Recombinant full-length human Nucleolin protein (Uniprot: P19338)

Does not react with Mouse, Feline, Bovine, Canine or Equine.

Nucleolus

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Marker of Human Cells

Monoclonal

Rabbit

IgG Kappa

Protein A or G purified

50mM Sodium Borate

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C in the dark.