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Nucleolin Antibody (NCL/8068R) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24068B

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-24068B

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # NCL/8068R

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Nucleolin Antibody (NCL/8068R) [Biotin]

Immunogen

Recombinant full-length human Nucleolin protein

Localization

Nucleoli

Specificity

This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Marker

Marker of Human Cells

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Applications for Nucleolin Antibody (NCL/8068R) [Biotin]

Application
Recommended Usage

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Nucleolin

Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. Nucleolin antibodies are commonly used as a mammalian nucleolus marker.

Alternate Names

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Gene Symbol

NCL

Additional Nucleolin Products

Product Documents for Nucleolin Antibody (NCL/8068R) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nucleolin Antibody (NCL/8068R) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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