Nucleolin Antibody (NCL/902) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47860

Novus Biologicals, part of Bio-Techne
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NBP2-47860-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NCL/902

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Nucleolin Antibodies »

Product Summary for Nucleolin Antibody (NCL/902) - Azide and BSA Free

Immunogen

Recombinant full-length human Nucleolin protein (Uniprot: P19338)

Reactivity Notes

Does not react with Mouse, Rat or Bovine.

Localization

Nucleoli

Specificity

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This Mouse Monoclonal Antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Marker

Marker of Human Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44610).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Nucleolin Antibody (NCL/902) - Azide and BSA Free

Western Blot: Nucleolin Antibody (NCL/902)Azide and BSA Free [NBP2-47860]

Western Blot: Nucleolin Antibody (NCL/902)Azide and BSA Free [NBP2-47860]

Western Blot: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860]

Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860]

Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - HeLa cells stained with AF488 labeled Nucleolin Antibody (NCL/902) Green: AF488-labeled Ab. Blue: DAPI.
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Formalin-fixed, paraffin-embedded human colon stained with Nucleolin Antibody (NCL/902).
View 8 more images

Applications for Nucleolin Antibody (NCL/902) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml

Western Blot

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Nucleolin

Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. Nucleolin antibodies are commonly used as a mammalian nucleolus marker.

Alternate Names

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Gene Symbol

NCL

Additional Nucleolin Products

Product Documents for Nucleolin Antibody (NCL/902) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nucleolin Antibody (NCL/902) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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