Nucleolin Antibody

Western Blot: Nucleolin Antibody [NB600-241]

Western Blot: Nucleolin Antibody [NB600-241] - Detection of nucleolin in crude PD31 nuclear extracts.

Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody [NB600-241]

Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). Image from verified customer review.

Immunohistochemistry: Nucleolin Antibody [NB600-241]

Immunohistochemistry: Nucleolin Antibody [NB600-241] - Detection of Nucleolin in human tonsil germinal center.

Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody [NB600-241]

Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - In-situ immunofluorescent staining of PD31 murine pre-B cells.

Western Blot: Nucleolin Antibody [NB600-241] -

Western Blot: Nucleolin Antibody [NB600-241] - HT-LKO cells recapitulate the hallmarks of lamin A/C deficiency.(a) Quantitative PCR shows a >6-fold reduction (expressed as log2 fold change of ddCt value) of LMNA transcripts in different LKO colonies as compared to HT-WT clones; (b) Quantitative immunofluorescence shows a dramatic reduction of A-type lamin levels in HT-LKO colonies, approximating background levels (dotted white line); (c) Western blot for lamin A/C reveals the absence of both proteins in HT-LKO cells; (d) Immunostaining & nuclear counterstaining of HT-LKO cells reveals their aberrant nuclear morphology, virtual absence of lamin A (green) & local depletion of lamin B (red) (arrowheads) as opposed to HT-WT cells; (e) Time-lapse recordings after H2B-GFP transfection illustrate increased nuclear plasticity of HT-LKO vs. HT-WT cells, as evidenced by their larger projected area (Σ Area) & contour changes (Σ Outline) across time; (f) HT-LKO nuclei have a lower average nuclear circularity (dotted white line indicates a circularity of 0.85) & higher nuclear circularity fluctuations (average + 95% confidence interval) than HT-WT cells; The Y-axis has been cropped for clarity; (g) This translates into a significantly larger coefficient of variation (CoV) for the circularity across time (p < 0.001). (h) Nuclear ruptures occur more frequently in HT-LKO cells than in HT-WT cells (p < 0.001); (i) Representative montages of the NLS channel of a C4 HT-WT & L2 HT-LKO cell, with corresponding NLS/H2B ratio measurements (grey-coded bar plots). The moments of rupture events are indicated as red dots. Bar graphs reflect mean ± standard error (n = number of tracks). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27461848), licensed under a CC-BY license. Not internally tested by Novus Biologicals.