Nucleostemin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1569

Novus Biologicals, part of Bio-Techne
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NB100-1569
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Nucleostemin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 549) of human Guanine Nucleotide Binding Protein-Like 3 (Nucleostemin) using the numbering given in entry NP_055181.3 (GeneID 26354).

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nucleostemin Antibody

Western Blot: Nucleostemin Antibody [NB100-1569]

Western Blot: Nucleostemin Antibody [NB100-1569]

Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by western blot. Samples:Whole cell lysate (50 ug) from TMCK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (NB100-1569). Detection: DAB
Western Blot: Nucleostemin Antibody [NB100-1569]

Western Blot: Nucleostemin Antibody [NB100-1569]

Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Applications for Nucleostemin Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nucleostemin

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Alternate Names

GNL3

Entrez Gene IDs

26354 (Human); 30877 (Mouse)

Gene Symbol

GNL3

UniProt

Q9BVP2 (Human)

Additional Nucleostemin Products

Product Documents for Nucleostemin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nucleostemin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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