Nucleostemin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38326

Novus Biologicals, part of Bio-Techne
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NBP2-38326
NBP2-38326-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nucleostemin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nucleostemin Antibody

Western Blot: Nucleostemin Antibody [NBP2-38326]

Western Blot: Nucleostemin Antibody [NBP2-38326]

Western Blot: Nucleostemin Antibody [NBP2-38326] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/ Immunofluorescence: Nucleostemin Antibody [NBP2-38326]

Immunocytochemistry/ Immunofluorescence: Nucleostemin Antibody [NBP2-38326]

Immunocytochemistry/Immunofluorescence: Nucleostemin Antibody [NBP2-38326] - Staining of human cell line U-2 OS shows localization to nucleoli & nuclear bodies.
Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326]

Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326]

Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human testis.
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Applications for Nucleostemin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nucleostemin

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Alternate Names

GNL3

Gene Symbol

GNL3

UniProt

Q9BVP2 (Human)

Additional Nucleostemin Products

Product Documents for Nucleostemin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nucleostemin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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