Nucleostemin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98743

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nucleostemin Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Nucleostemin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Nucleostemin Antibody

Western Blot: Nucleostemin Antibody [NBP2-98743]

Western Blot: Nucleostemin Antibody [NBP2-98743]

Western Blot: Nucleostemin Antibody [NBP2-98743] - Anti-Nucleostemin rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: Nucleostemin Antibody [NBP2-98743]

Immunocytochemistry/ Immunofluorescence: Nucleostemin Antibody [NBP2-98743]

Immunocytochemistry/Immunofluorescence: Nucleostemin Antibody [NBP2-98743] - Immunofluorescence staining of Nucleostemin in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Nucleostemin polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green)Positive staining was localized to nucleus.

Applications for Nucleostemin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:5000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nucleostemin

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Alternate Names

GNL3

Gene Symbol

GNL3

Additional Nucleostemin Products

Product Documents for Nucleostemin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nucleostemin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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