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NUDC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51971

Novus Biologicals, part of Bio-Techne
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NBP1-51971

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-PNLGNGADLPNYR corresponding to internal region according to NP_006591.1.

Epitope

C-PNLGNGADLPNYR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NUDC Antibody

Western Blot: NUDC Antibody [NBP1-51971]

Western Blot: NUDC Antibody [NBP1-51971]

Western Blot: NUDC Antibody [NBP1-51971] - (0.5ug/ml) staining of HeLa (A), HepG2 (B), K562 (C) and KNRK (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: NUDC Antibody [NBP1-51971]

Immunocytochemistry/ Immunofluorescence: NUDC Antibody [NBP1-51971]

Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-51971] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for NUDC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx 40kDa band observed in lysates of cell lines HeLa. HepG2 and K562, and approx. 38kDa in lysates of cell line KNRK (calculated MW of 38.2kDa according to Human NP_006591.1) and 38.4kDa according to Rat NP_058967.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NUDC

NudC was first identified as a regulator of nuclear movement in the asexual reproductive cycle of the filamentous fungus Aspergillus nidulans. Human NUDC is a nuclear movement protein that associates with dynein (see DYNC1H1; MIM 600112) (Aumais et al., 2003 [PubMed 12679384]).[supplied by OMIM]

Alternate Names

HNUDC, MNUDC, NPD011, nuclear distribution gene C (A.nidulans) homolog, nuclear distribution gene C homolog (A. nidulans), Nuclear distribution protein C homolog, nuclear migration protein nudC, NudC

Entrez Gene IDs

10726 (Human); 18221 (Mouse); 29648 (Rat)

Gene Symbol

NUDC

Additional NUDC Products

Product Documents for NUDC Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NUDC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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