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NUDT21 Antibody (3F8)

Novus Biologicals, part of Bio-Techne | Catalog # H00011051-M12

Novus Biologicals, part of Bio-Techne
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H00011051-M12

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NUDT21 (AAH01403, 1 a.a. ~ 227 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN

Reactivity Notes

Human. Other species not tested.

Specificity

NUDT21 (3F8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NUDT21 Antibody (3F8)

Immunocytochemistry/ Immunofluorescence: NUDT21 Antibody (3F8) [H00011051-M12]

Immunocytochemistry/ Immunofluorescence: NUDT21 Antibody (3F8) [H00011051-M12]

Immunocytochemistry/Immunofluorescence: NUDT21 Antibody (3F8) [H00011051-M12] - Analysis of monoclonal antibody to NUDT21 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: NUDT21 Antibody (3F8) [H00011051-M12]

Immunohistochemistry-Paraffin: NUDT21 Antibody (3F8) [H00011051-M12]

Immunohistochemistry-Paraffin: NUDT21 Antibody (3F8) [H00011051-M12] - Analysis of monoclonal antibody to NUDT21 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml
ELISA: NUDT21 Antibody (3F8) [H00011051-M12]

ELISA: NUDT21 Antibody (3F8) [H00011051-M12]

ELISA: NUDT21 Antibody (3F8) [H00011051-M12] - Detection limit for recombinant GST tagged NUDT21 is approximately 3ng/ml as a capture antibody.

Applications for NUDT21 Antibody (3F8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NUDT21

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides.

Alternate Names

CFIm25, CFIM25CPSF 25 kDa subunit, cleavage and polyadenylation specific factor 5, 25 kD subunit, cleavage and polyadenylation specific factor 5, 25 kDa, Cleavage and polyadenylation specificity factor 25 kDa subunit, cleavage and polyadenylation specificity factor subunit 5, CPSF25, CPSF5DKFZp686H1588, Nucleoside diphosphate-linked moiety X motif 21, nudix (nucleoside diphosphate linked moiety X)-type motif 21, Nudix motif 21, pre-mRNA cleavage factor Im (25kD), Pre-mRNA cleavage factor Im 25 kDa subunit, pre-mRNA cleavage factor Im, 25kD subunit

Entrez Gene IDs

11051 (Human)

Gene Symbol

NUDT21

OMIM

604978 (Human)

Additional NUDT21 Products

Product Documents for NUDT21 Antibody (3F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NUDT21 Antibody (3F8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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