NuMA Antibody (6D4I4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16411

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16411-100ul
NBP3-16411-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6D4I4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NuMA Antibodies »

Product Summary for NuMA Antibody (6D4I4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2000-2100 of human NuMA1 (Q14980). TPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NuMA Antibody (6D4I4)

Western Blot: NuMA Antibody (6D4I4) [NBP3-16411]

Western Blot: NuMA Antibody (6D4I4) [NBP3-16411]

Western Blot: NuMA Antibody (6D4I4) [NBP3-16411] - Western blot analysis of extracts of various cell lines, using NuMA1 Rabbit mAb (NBP3-16411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: NuMA Antibody (6D4I4) [NBP3-16411]

Immunohistochemistry-Paraffin: NuMA Antibody (6D4I4) [NBP3-16411]

Immunohistochemistry-Paraffin: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry of paraffin-embedded mouse brain using NuMA1 Rabbit mAb (NBP3-16411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: NuMA Antibody (6D4I4) [NBP3-16411]

Immunohistochemistry-Paraffin: NuMA Antibody (6D4I4) [NBP3-16411]

Immunohistochemistry-Paraffin: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry of paraffin-embedded rat brain using NuMA1 Rabbit mAb (NBP3-16411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for NuMA Antibody (6D4I4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NuMA

NuMA (nuclear mitotic apparatus) is a long coiled-coil protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker.

Long Name

Nuclear Mitotic Apparatus Protein 1

Alternate Names

NMP-22, NUMA1, SP-H Antigen

Gene Symbol

NUMA1

Additional NuMA Products

Product Documents for NuMA Antibody (6D4I4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NuMA Antibody (6D4I4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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