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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1699 and 1749 of human nucleoporin 188kDa using the numbering given in entry NP_056169.1 (GeneID 23511).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NUP188 Antibody

Western Blot: NUP188 Antibody [NBP1-28717]

Western Blot: NUP188 Antibody [NBP1-28717]

Western Blot: NUP188 Antibody [NBP1-28717] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUP188 antibody NBP1-28717 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NUP188 was also immunoprecipitated by rabbit anti-NUP188 antibody NBP1-28748, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for NUP188 Antibody

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NUP188

Nucleoporin 188 kDa (NUP188) is one of many nucleoporin proteins that make up the nucleoporin complex which function reside in the nuclear membrane to form nuclear pore structures.

Alternate Names

FLJ21639, KIAA0169hNup188, nucleoporin 188kDa, nucleoporin NUP188 homolog

Entrez Gene IDs

23511 (Human)

Gene Symbol

NUP188

Additional NUP188 Products

Product Documents for NUP188 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NUP188 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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