NUP188 Antibody

Western Blot: NUP188 Antibody [NBP1-28748]

Western Blot: NUP188 Antibody [NBP1-28748] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUP188 antibody NBP1-28748 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NUP188 was also immunoprecipitated by rabbit anti-NUP188 antibody NBP1-28717, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Western Blot: NUP188 Antibody [NBP1-28748] -

Western Blot: NUP188 Antibody [NBP1-28748] - Full induction of p21 is Nup155-dependent. a Protein fold-changes (blue & grey dots) including p53 targets (red dots) upon Nup155 (orange dot) knockdown compared to the control siRNA (AS) condition are shown as log2 ratios. p21 is highlighted in green as a key effector of the p53 response. Overall 3523 proteins could be quantified by quantitative mass spectrometry in HepG2 cells treated with Nutlin-3a for 24 h. b HepG2 cells were treated either with control siRNA (AS) or three different Nup155 siRNAs (Nup155#1, Nup155#2, & Nup155#3) for 72 h & p53 was induced by adding Nutlin-3a for 24 h. Cell extracts were analysed by immunoblotting with indicated antibodies (upper panel). Densitometric quantification of immunoblots derived from three independent experiments is shown in the lower panel & normalised to the control siRNA (AS) condition. c HepG2 cells were treated either with control siRNA (AS) or two different Nup35, Nup93, & Nup188 siRNAs (Nup35#1, Nup35#2, Nup93#1, Nup93#2, Nup188#1, & Nup188#2) for 72 h. Cells were harvested upon 24 h of Nutlin-3a treatment & extracts were analysed by immunoblotting with the indicated antibodies. For corresponding densitometric analyses see Supplementary Figure 1B-D. d Protein fold-changes of NPC components (purple dots) upon Nup155 knockdown (orange dot) corresponding to the conditions described in a. e Representative confocal microscopy pictures of MAb414 immunofluorescence staining of HepG2 cells treated as indicated. DAPI was used for DNA labeling. Scale bar = 10 µm. *p < 0.05, **p < 0.01, ***p < 0.001 (Student′s t-test). Data are presented as mean ± stdv. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31089132), licensed under a CC-BY license. Not internally tested by Novus Biologicals.