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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for NUP205 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1750 and 1800 of human Nucleoporin 205kDa using the numbering given in entry NP_055950.1 (GeneID 23165).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NUP205 Antibody

Western Blot: NUP205 Antibody [NBP2-22287]

Western Blot: NUP205 Antibody [NBP2-22287]

Western Blot: NUP205 Antibody [NBP2-22287] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: NBP2-22287 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: NUP205 Antibody [NBP2-22287]

Immunoprecipitation: NUP205 Antibody [NBP2-22287]

Immunoprecipitation: NUP205 Antibody [NBP2-22287] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22287 used for IP at 6 ug/mg lysate. NUP205 was also immunoprecipitated by rabbit anti-NUP205 antibody. For blotting immunoprecipitated NUP205, NBP2-22287 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for NUP205 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NUP205

Alternate Names

nucleoporin 205kDa

Gene Symbol

NUP205

Additional NUP205 Products

Product Documents for NUP205 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NUP205 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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