NUP50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1212

Novus Biologicals, part of Bio-Techne
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NB100-1212
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NUP50 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ELHKILLEKKDA corresponding to C-Terminus according to NP_009103.2, NP_705931.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NUP50 Antibody

Western Blot: NUP50 Antibody [NB100-1212]

Western Blot: NUP50 Antibody [NB100-1212]

Western Blot: NUP50 Antibody [NB100-1212] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-1212]

Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-1212]

Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-1212] - 3.8ug/ml staining of Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NUP50 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.2 - 1 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in Jurkat lysates (calculated MW 50.1 kDa band according to NP_009103.2). IHC-P: Human uterus shows textured nuclear staining of endometrial cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NUP50

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

50 kDa nucleoporin, MGC39961, NPAP60, NPAP60LNucleoporin Nup50, nuclear pore complex protein Nup50, Nuclear pore-associated protein 60 kDa-like, nuclear pore-associated protein 60L, nucleoporin 50kD, nucleoporin 50kDa

Entrez Gene IDs

10762 (Human); 18141 (Mouse)

Gene Symbol

NUP50

UniProt

NP_009103 (Human)

Additional NUP50 Products

Product Documents for NUP50 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NUP50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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