NUP50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93324

Novus Biologicals, part of Bio-Techne
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NB100-93324
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all NUP50 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 418 and 468 of human nucleoporin 50kDa using the numbering given in entry NP_009103.2 (GeneID 10762).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NUP50 Antibody

Western Blot: NUP50 Antibody [NB100-93324]

Western Blot: NUP50 Antibody [NB100-93324]

Western Blot: NUP50 Antibody [NB100-93324] - MDA-MB-231 nuclear extract. Cells infected with lentivirus coding scramble shRNA or one of two NUP50 targeting shRNAs. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-93324]

Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-93324]

Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-93324] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- NUP50 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: NUP50 Antibody [NB100-93324]

Western Blot: NUP50 Antibody [NB100-93324]

Western Blot: NUP50 Antibody [NB100-93324] - Detection of Human NUP50 on HeLa whole cell lysate using NB100-93324. NUP50 was also immunoprecipitated by rabbit anti-NUP50 antibody NB100-93323.

Applications for NUP50 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. NUP50 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-93324 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NUP50

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

50 kDa nucleoporin, MGC39961, NPAP60, NPAP60LNucleoporin Nup50, nuclear pore complex protein Nup50, Nuclear pore-associated protein 60 kDa-like, nuclear pore-associated protein 60L, nucleoporin 50kD, nucleoporin 50kDa

Entrez Gene IDs

10762 (Human)

Gene Symbol

NUP50

UniProt

Q9UKX7 (Human)

Additional NUP50 Products

Product Documents for NUP50 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NUP50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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