NUP50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13683

Novus Biologicals, part of Bio-Techne
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NBP2-13683
NBP2-13683-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NUP50 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSQQPSSSGLASSKACVGNAYHKQLAALDCSVRD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NUP50 Antibody

Western Blot: NUP50 Antibody [NBP2-13683]

Western Blot: NUP50 Antibody [NBP2-13683]

Western Blot: NUP50 Antibody [NBP2-13683] - Analysis using Anti-NUP50 antibody NBP2-13683 (A) shows similar pattern to independent antibody NBP2-57627 (B).
Immunocytochemistry/ Immunofluorescence: NUP50 Antibody [NBP2-13683]

Immunocytochemistry/ Immunofluorescence: NUP50 Antibody [NBP2-13683]

Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-13683] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear membrane.
Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-13683]

Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-13683]

Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-13683] - Staining in human testis and heart muscle tissues using anti-NUP50 antibody. Corresponding NUP50 RNA-seq data are presented for the same tissues.
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Applications for NUP50 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NUP50

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

50 kDa nucleoporin, MGC39961, NPAP60, NPAP60LNucleoporin Nup50, nuclear pore complex protein Nup50, Nuclear pore-associated protein 60 kDa-like, nuclear pore-associated protein 60L, nucleoporin 50kD, nucleoporin 50kDa

Gene Symbol

NUP50

Additional NUP50 Products

Product Documents for NUP50 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NUP50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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