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NUP50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19610

Novus Biologicals, part of Bio-Techne
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NBP2-19610

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NUP50. The exact sequence is proprietary.

Localization

Nucleus; nuclear pore complex; Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NUP50 Antibody

Immunocytochemistry/ Immunofluorescence: NUP50 Antibody [NBP2-19610]

Immunocytochemistry/ Immunofluorescence: NUP50 Antibody [NBP2-19610]

Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-19610] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUP50 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2500.
Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-19610]

Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-19610]

Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-19610] - Immunohistochemical analysis of paraffin-embedded Normal colon, using antibody at 1:250 dilution.
Western Blot: NUP50 Antibody [NBP2-19610]

Western Blot: NUP50 Antibody [NBP2-19610]

Western Blot: NUP50 Antibody [NBP2-19610] - 50 ug mouse testis lysate/extract 10% SDS-PAGE NUP50 antibody [C3], C-term dilution: 1:1000.

Applications for NUP50 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NUP50

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

50 kDa nucleoporin, MGC39961, NPAP60, NPAP60LNucleoporin Nup50, nuclear pore complex protein Nup50, Nuclear pore-associated protein 60 kDa-like, nuclear pore-associated protein 60L, nucleoporin 50kD, nucleoporin 50kDa

Gene Symbol

NUP50

UniProt

Additional NUP50 Products

Product Documents for NUP50 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NUP50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.