NUP62 Antibody (JE55-09)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32670
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE55-09
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human NUP62 aa 483-522. (Uniprot: P37198)
Localization
Nucleus, nuclear pore complex, Cytoplasm, cytoskeleton, spindle pole, Nucleus envelope, microtubule organizing center, centrosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NUP62 Antibody (JE55-09)
Western Blot: NUP62 Antibody (JE55-09) [NBP3-32670]
Western Blot: NUP62 Antibody (JE55-09) [NBP3-32670] - Western blot analysis of NUP62 on different lysates with Rabbit anti-NUP62 antibody (NBP3-32670) at 1/1,000 dilution.Lane 1: Jurkat cell lysate
Lane 2: A431 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Raji cell lysate
Lane 5: HepG2 cell lysate
Lane 6: C2C12 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: PC-12 cell lysate
Lane 9: NIH/3T3 cell lysate
Lane 10: Neuro-2a cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 53 kDa
Observed band size: 61 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32670) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: NUP62 Antibody (JE55-09) [NBP3-32670]
Immunohistochemistry: NUP62 Antibody (JE55-09) [NBP3-32670] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUP62 antibody (NBP3-32670) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32670) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for NUP62 Antibody (JE55-09)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NUP62
Alternate Names
DKFZp547L134,62 kDa nucleoporin, FLJ20822, FLJ43869, IBSNp62, MGC841, nuclear pore glycoprotein p62, nucleoporin 62kD, nucleoporin 62kDa, SNDINucleoporin Nup62
Gene Symbol
NUP62
Additional NUP62 Products
Product Documents for NUP62 Antibody (JE55-09)
Product Specific Notices for NUP62 Antibody (JE55-09)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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