NUP98 Antibody (2H4)

Western Blot: NUP98 Antibody (2H4) [NBP3-15200]

Western Blot: NUP98 Antibody (2H4) [NBP3-15200] - Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate. All lanes: NUP98 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 198, 188, 98, 97, 196, 187 KDa. Observed band size: 100 KDa.

Immunocytochemistry/ Immunofluorescence: NUP98 Antibody (2H4) [NBP3-15200]

Immunocytochemistry/Immunofluorescence: NUP98 Antibody (2H4) [NBP3-15200] - Immunofluorescence staining of Hela Cells with at NUP98 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry: NUP98 Antibody (2H4) [NBP3-15200]

Immunohistochemistry: NUP98 Antibody (2H4) [NBP3-15200] - IHC image of NUP98 antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.