O-GlcNAc Antibody (HGAC85)

Novus Biologicals, part of Bio-Techne | Catalog # NB300-614

Novus Biologicals, part of Bio-Techne
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NB300-614
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, All Species, Primate

Cited:

Human, Mouse, Rat, Primate

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # HGAC85

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all O-GlcNAc Antibodies »

Product Specifications

Immunogen

Heat-killed, pepsin-treated group A streptococci (Streptococcus pyogenes)

Reactivity Notes

All Species. Human reactivity reported in scientific literature (PMID: 28973823). Chlorocebus sabaeus (Green monkey) reported in PMID 24256719.

Specificity

Recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for O-GlcNAc Antibody (HGAC85)

Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614]

Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614]

Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614] - alpha-DG-Fc was transiently transfected with or without AGO61 into COS1 cells. alpha-DG-Fc recombinant proteins were collected from cell lysates and culture media using protein A resin and analyzed for laminin overlay and Western blot using anti-Fc and anti-O-GlcNAc antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/srep03288), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: O-GlcNAc Antibody (HGAC85) [NB300-614]

Immunohistochemistry-Paraffin: O-GlcNAc Antibody (HGAC85) [NB300-614]

Immunohistochemistry-Paraffin: O-GlcNAc Antibody (HGAC85) [NB300-614] - IHC staining of O-GlcNAc in mouse colon using DAB with hematoxylin counterstain.
Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614]

Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614]

Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614] - Western blot analysis of O-GlcNAc expression in 1) HeLa, 2) NTERA-2, 3) PC-12 and 4) COS-7 cell lysates using untreated antibody on the left side and antibody preincubated with 50 uM GlcNAc (see Notes).
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Applications for O-GlcNAc Antibody (HGAC85)

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10-1:500. Use reported in scientific literature (PMID 20368426)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200. Use reported in scientific literature (Hamiel CR et al)

Immunohistochemistry

1:200. Use reported in scientific literature (Zhang , et al)

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:500. Use reported in scientific literature (Hamiel CR et al)

Western Blot

1:500-1:1000
Application Notes
In Western blot, this antibody detects several proteins representing O-GlcNAc glycoproteins. Immunofluorescence staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties. DO NOT USE WITH DILUENTS CONTAINING GLYCOSYLATED PROTEINS.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: O-GlcNAc

O-GlcNAc (O-linked N-acetylglucosamine) is one of the most abundant posttranslational modifications on nuclear and cytoplasmic proteins. Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-GlcNAc residues attached to serine or threonine residues. This modification occurs via the O-GlcNAc transferase (OGT). There appears to be a competitive modification at these residues. Either the residues are glycosylated or they are phosphorylated. Because of the significance of phosphorylation in cancer research, such competition can be of major interest. O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may also obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling. Recent research has revealed that the O-GlcNAcylation of the EGF repeats of Notch are modified via a novel O-GlcNAc transferase, EOGT1 (PMID: 22310717).

Alternate Names

GlcNAc, O-linked N-acetylglucosamine

Additional O-GlcNAc Products

Product Documents for O-GlcNAc Antibody (HGAC85)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for O-GlcNAc Antibody (HGAC85)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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