O-GlcNAc Antibody (RL2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80892
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, Drosophila, Fish, Hamster, Primate, Virus, Xenopus
Applications
Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), CyTOF-ready, Dot Blot, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # RL2
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID: 26004176). Xenopus reactivity reported in scientific literature (PMID: 17329255). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Cytoplasmic and Nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for O-GlcNAc Antibody (RL2) - Azide and BSA Free
Western Blot: O-GlcNAc Antibody (RL2)Azide and BSA Free [NBP2-80892]
Western Blot: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - Analysis of mouse cortical brain lysates using O-Linked N-Acetylglucosamine Monoclonal Antibody. Blots containing cortical extracts from 4 individual C57BL/6 mice (Lanes 1-4) were blocked with 5% milk in TBST, and probed with MA1-072 at 1:1000, followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody. Data courtesy of the Innovators Program. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]
Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-O-GlcNAc (RL2) at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.Immunohistochemistry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]
Immunohistochemistry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - Analysis of a FFPE tissue section of the mouse colon using 1:200 dilution of O-GlcNAc [RL2] antibody (NB300-524). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. Image from the standard format ofApplications for O-GlcNAc Antibody (RL2) - Azide and BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation
1:10 - 1:500. Use reported in scientific literature (PMID 20404350)
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Dot Blot
1:800
ELISA
1:100 - 1:2000,. Use reported in scientific literature (PMID 12029848)
Flow Cytometry
1:10 - 1:1000
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
1:10 - 1:500
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: O-GlcNAc
Alternate Names
GlcNAc, O-linked N-acetylglucosamine
Additional O-GlcNAc Products
Product Documents for O-GlcNAc Antibody (RL2) - Azide and BSA Free
Product Specific Notices for O-GlcNAc Antibody (RL2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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