O-GlcNAc Transferase p110 subunit Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52925

Novus Biologicals, part of Bio-Techne
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NBP1-52925
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all O-GlcNAc Transferase/OGT Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to OGT(O-linked N-acetylglucosamine (GlcNAc) transferase) The peptide sequence was selected from the N terminal of OGT. Peptide sequence ASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQE. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for O-GlcNAc Transferase p110 subunit Antibody

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-52925]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-52925]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-52925] - Analysis of 721_B whole cell lysates, Antibody Dilution: 1.0 ug/ml OGT is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-52925]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-52925]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-52925] - Formalin Fixed Paraffin; Embedded Tissue: Human Pineal Tissue; Observed Staining: Cytoplasmic in processes of pinealocytes and endothelial cells in blood vessels; Primary Antibody Concentration: 1:100

Applications for O-GlcNAc Transferase p110 subunit Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: O-GlcNAc Transferase/OGT

OGT catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

O-Linked N-Acetylglucosamine (GlcNAc) Transferase

Alternate Names

HRNT1, OGlcNAc Transferase, OGT

Gene Symbol

OGT

UniProt

O15294

Additional O-GlcNAc Transferase/OGT Products

Product Documents for O-GlcNAc Transferase p110 subunit Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for O-GlcNAc Transferase p110 subunit Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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