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O-GlcNAcase/OGA/MGEA5 Antibody (8D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26142

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26142-100ul
NBP3-26142-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8D12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human O-GlcNAcase/OGA/MGEA5 [UniProt O60502]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for O-GlcNAcase/OGA/MGEA5 Antibody (8D12)

O-GlcNAcase/OGA/MGEA5 Antibody (8D12)

Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody (8D12) [NBP3-26142] -

Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody (8D12) [NBP3-26142] - Positive Western Blot detected in: Hela whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate.
All lanes: O-GlcNAcase/OGA/MGEA5 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 103, 96, 77, 97 kDa
Observed band size: 130 kDa

Applications for O-GlcNAcase/OGA/MGEA5 Antibody (8D12)

Application
Recommended Usage

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: O-GlcNAcase/OGA

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Alternate Names

GH84, HEXC3, Hexosaminidase B, MGEA5, NCOAT, OGA, OGlcNAcase

Gene Symbol

OGA

Additional O-GlcNAcase/OGA Products

Product Documents for O-GlcNAcase/OGA/MGEA5 Antibody (8D12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for O-GlcNAcase/OGA/MGEA5 Antibody (8D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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