Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all O-GlcNAcase/OGA Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 500 to 550 of human Meningioma Expressed Antigen 5 using the numbering given in entry NP_036347.1 (GeneID 10724).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for O-GlcNAcase/OGA/MGEA5 Antibody

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Section of human testicular seminoma. Antibody: Affinity purified rabbit anti- MGEA5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]

Immunoprecipitation: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]

Immunoprecipitation: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MGEA5 antibody NBP2-32233 used for IP at 6 ug per reaction. MGEA5 was also immunoprecipitated by rabbit anti-MGEA5 antibody BL15772. For blotting immunoprecipitated MGEA5, NBP2-32233 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
O-GlcNAcase/OGA/MGEA5 Antibody

Western Blot- O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] -

Western Blot- O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Human MGEA5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MGEA5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for O-GlcNAcase/OGA/MGEA5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: O-GlcNAcase/OGA

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Alternate Names

GH84, HEXC3, Hexosaminidase B, MGEA5, NCOAT, OGA, OGlcNAcase

Gene Symbol

OGA

UniProt

NP_036347.1 (Human)

Additional O-GlcNAcase/OGA Products

Product Documents for O-GlcNAcase/OGA/MGEA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for O-GlcNAcase/OGA/MGEA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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