Occludin Antibody (5A7)
Novus Biologicals, part of Bio-Techne | Catalog # H00004950-M02
Key Product Details
Species Reactivity
Validated:
Human, Rat, Porcine
Cited:
Rat
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5A7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
OCLN (NP_002529, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
Reactivity Notes
Use in Rat reported in scientific literature (PMID:33460804)
Specificity
This product is specific for Human OCLN monoclonal antibody (M02), clone 5A7 [Gene ID: 4950].
Marker
Tight Junctions Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Occludin Antibody (5A7)
![Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]](https://resources.bio-techne.com/images/products/Occludin-Antibody-5A7-Immunocytochemistry-Immunofluorescence-H00004950-M02-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]")
Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]
Immunocytochemistry/Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02] - Occludin labeled Pig SC cells visualized by Alexa 488 conjugated secondary antibody. This image was submitted via customer Review.![Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]](https://resources.bio-techne.com/images/products/Occludin-Antibody-5A7-Immunocytochemistry-Immunofluorescence-H00004950-M02-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]")
Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]
Immunocytochemistry/Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02] - Analysis of monoclonal antibody to OCLN on HeLa cell . Antibody concentration 10 ug/ml.![ELISA: Occludin Antibody (5A7) [H00004950-M02]](https://resources.bio-techne.com/images/products/Occludin-Antibody-5A7-ELISA-H00004950-M02-img0004.jpg "ELISA: Occludin Antibody (5A7) [H00004950-M02]")
ELISA: Occludin Antibody (5A7) [H00004950-M02]
ELISA: Occludin Antibody (5A7) [H00004950-M02] - Detection limit for recombinant GST tagged OCLN is approximately 1ng/ml as a capture antibody.Applications for Occludin Antibody (5A7)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using H00004950-M02 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Occludin
Additional Occludin Products
Product Documents for Occludin Antibody (5A7)
Product Specific Notices for Occludin Antibody (5A7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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