Occludin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77037

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Occludin Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN. The immunogen is located within the last 50 amino acids of OCLN. Amino Acid Squence: SHIKKMVGDYDRQKT

Specificity

At least three isoforms of OCLN are known to exist.

Marker

Tight Junctions Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Occludin Antibody - BSA Free

Western Blot: Occludin AntibodyBSA Free [NBP1-77037]

Western Blot: Occludin AntibodyBSA Free [NBP1-77037]

Western Blot: Occludin Antibody [NBP1-77037] - Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 ug/mL.
Occludin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Occludin Antibody - BSA Free [NBP1-77037] -

Immunocytochemistry/ Immunofluorescence: Occludin Antibody - BSA Free [NBP1-77037] - Immunofluorescence of Occludin in mouse brain tissue with Occludin antibody at 20 ug/mL.

Red: Occludin Antibody
Blue: DAPI staining
Occludin Antibody - BSA Free

Immunohistochemistry: Occludin Antibody - BSA Free [NBP1-77037] -

Immunohistochemistry: Occludin Antibody - BSA Free [NBP1-77037] - Immunohistochemistry of Occludin in mouse brain tissue with Occludin Antibody at 5 ug/mL.
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Applications for Occludin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Occludin

Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.

Alternate Names

BLCPMG, OCLN

Entrez Gene IDs

100506658 (Human); 83497 (Rat)

Gene Symbol

OCLN

UniProt

Q16625

Additional Occludin Products

Product Documents for Occludin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Occludin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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