OCIL/CLEC2d Antibody (166C1309)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56553

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 166C1309

Concentration

0.5 mg/ml

View all OCIL/CLEC2d Antibodies »

Product Specifications

Immunogen

This antibody was developed by immunizing mice with a synthetic peptide containing amino acids 2-18 (CVTKASLPMLSPTGSPQ) of mouse OCIL, GenBank no. gb|AAL37198.1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for OCIL/CLEC2d Antibody (166C1309)

Western Blot: OCIL/CLEC2d Antibody (166C1309) [NB100-56553]

Western Blot: OCIL/CLEC2d Antibody (166C1309) [NB100-56553]

Western Blot: OCIL/CLEC2d Antibody (166C1309) [NB100-56553] - Analysis of OCIL in mouse spleen cell lysates. Thirty microgram of cell lysates per lane of mini gel.

Applications for OCIL/CLEC2d Antibody (166C1309)

Application
Recommended Usage

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OCIL/CLEC2d

Recently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin (OCIL) can also inhibit osteoclast formation in spleen cell cultures treated with RANKL and M-CSF. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its inhibitory action on adherent spleen cell cultures, which were depleted of stromal and lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase of osteoclast formation and resulted in 70% inhibition during the differentiation phase. Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol, interleukin-1 and IL-11, and retinoic acid. OCIL is expressed in osteoblasts and chondrocytes as well as in a variety of extraskeletal tissues. Since OCIL expression was found to be very similar to distribution for RANKL, it has been suggested that RANKl and OCIL may interact in skeleton and in extracellular tissues.

Long Name

Osteoclast Inhibitory Lectin

Alternate Names

CLEC2D, LLT1

Entrez Gene IDs

93694 (Mouse)

Gene Symbol

CLEC2D

UniProt

AAK50881 (Mouse)

Additional OCIL/CLEC2d Products

Product Documents for OCIL/CLEC2d Antibody (166C1309)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OCIL/CLEC2d Antibody (166C1309)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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