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Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Western Blot

Label

Janelia Fluor 549

Antibody Source

Monoclonal Rat IgG2B Clone # 240408

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860

Specificity

Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Oct-3/4 Antibody (240408) [Janelia Fluor® 549]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Oct-3/4

Oct-3/4 is a POU transcription factor that is expressed in embryonic stem (ES) cells and germ cells, and its expression is required to sustain cell self-renewal and pluripotency. Oct-3/4 is the most recognized marker for totipotent ES cells.

Long Name

Octamer-binding Protein 3/4

Alternate Names

3-Oct, Oct4, Otf3g, Otf4, Pou5f1

Gene Symbol

POU5F1

Additional Oct-3/4 Products

Product Documents for Oct-3/4 Antibody (240408) [Janelia Fluor® 549]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Oct-3/4 Antibody (240408) [Janelia Fluor® 549]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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