Oct-3/4 Antibody (EM92)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00352

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # EM92

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Oct-3/4 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is OCT3/4, Pou5f1, transcription factor 1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Oct-3/4 Antibody (EM92)

Western Blot: Oct-3/4 Antibody (EM92) [NBP2-00352]

Western Blot: Oct-3/4 Antibody (EM92) [NBP2-00352]

Western Blot: OCT3/4 Antibody (EM92) [NBP2-00352] - F9 mouse embryonal carcinoma cell line (left) and mouse splenocyte (right) lysates were loaded at 1x10e5 cells/lane, probed with 2 ug/mL of Anti-Mouse OCT3/4 Purified and revealed with Anti-Rat IgG HRP.

Applications for Oct-3/4 Antibody (EM92)

Application
Recommended Usage

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Oct-3/4

The EM92 monoclonal antibody reacts with mouse and human Oct-3/4, encoded by the Pou5F1 gene. Oct-3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct-3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct-3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct-3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct-3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).

Long Name

Octamer-binding Protein 3/4

Alternate Names

3-Oct, Oct4, Otf3g, Otf4, Pou5f1

Gene Symbol

POU5F1

Additional Oct-3/4 Products

Product Documents for Oct-3/4 Antibody (EM92)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Oct-3/4 Antibody (EM92)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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