Oct-4A Antibody (03)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12775

Novus Biologicals, part of Bio-Techne
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NBP3-12775-100ul
NBP3-12775-50ul
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 03

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Oct-4A Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to the C-terminus of Human Oct-4A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Oct-4A Antibody (03)

Flow Cytometry: Oct-4A Antibody (03) [NBP3-12775]

Flow Cytometry: Oct-4A Antibody (03) [NBP3-12775]

Flow Cytometry: Oct-4A Antibody (03) [NBP3-12775] - Analysis of Oct4 in human embryonic stem (ES) cells. Human embryonic stem (ES) cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization buffer and stained with Purified Mouse anti-Oct4 monoclonal antibody, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for Oct-4A Antibody (03)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Oct-4A

Oct-3/4, alternately Oct-3 or Oct-4, is POU5F1 (POU domain containing, class 5, transcription factor 1), a 360 amino acid (aa) transcription factor that is expressed in totipotent embryonic stem and germ cells. The human Oct-4, Oct-3/4 or POU5F1 gene can be transcribed into at least three transcripts (Oct-4A, Oct-4B, and Oct-4B1) and generates four protein isoforms by alternative splicing and alternative translation initiation. Oct-4A expression is restricted to embryonic stem (ES) and embryonic carcinoma (EC) cells and is believed to be the transcription factor responsible for the pluripotency properties of embryonic stem (ES) cells. In contrast, Oct-4B/4B1 can be detected in various nonpluripotent cell types and cannot sustain ES cell pluripotency and self-renewal.

Long Name

Octamer-binding Transcription Factor 4A

Alternate Names

Oct4A

Gene Symbol

POU5F1

Additional Oct-4A Products

Product Documents for Oct-4A Antibody (03)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Oct-4A Antibody (03)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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