OCT4 Antibody (486)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15052

Novus Biologicals, part of Bio-Techne
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NBP2-15052
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rabbit

Predicted:

Chimpanzee (100%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 486

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all OCT4 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Reactivity Notes

Feline (89%), Porcine (88%),Bovine (85%). Rabbit reactivity reported from a verified customer review.

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OCT4 Antibody (486)

Western Blot: OCT4 Antibody (486) [NBP2-15052]

Western Blot: OCT4 Antibody (486) [NBP2-15052]

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052]

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052]

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Paraffin-embedded human cervical carcinoma.Oct4 stained by Oct4 antibody [GT486] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Flow Cytometry: OCT4 Antibody (486) [NBP2-15052]

Flow Cytometry: OCT4 Antibody (486) [NBP2-15052]

Flow Cytometry: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody dilution: 1:50
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Applications for OCT4 Antibody (486)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:1000-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-15052 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol (pH7)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OCT4

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Alternate Names

class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein

Gene Symbol

POU5F1

Additional OCT4 Products

Product Documents for OCT4 Antibody (486)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for OCT4 Antibody (486)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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