OCT4 Antibody (OCT4/6875R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13745
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745]](https://resources.bio-techne.com/images/products/OCT4-Antibody-OCT4-6875R-Immunohistochemistry-Paraffin-NBP3-13745-img0002.jpg "Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # OCT4/6875R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human OCT4 protein (around aa 1-134) (exact sequence is proprietary) (Uniprot: Q01860)
Localization
Nucleus
Specificity
OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.
Marker
Germ Cell Tumor Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
38.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14148)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for OCT4 Antibody (OCT4/6875R)
Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745]
Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745] - Formalin-fixed, paraffin-embedded human testis stained with OCT4 antibody (OCT4/6875R). Inset: PBS instead of primary antibody, secondary negative control.![Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745]](https://resources.bio-techne.com/images/products/OCT4-Antibody-OCT4-6875R-Immunohistochemistry-Paraffin-NBP3-13745-img0001.jpg "Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745]")
Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745]
Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745] - Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.![Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745]](https://resources.bio-techne.com/images/products/OCT4-Antibody-OCT4-6875R-Immunohistochemistry-Paraffin-NBP3-13745-img0003.jpg "Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745]")
Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745]
Immunohistochemistry-Paraffin: OCT4 Antibody (OCT4/6875R) [NBP3-13745] - Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.Applications for OCT4 Antibody (OCT4/6875R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: OCT4
Alternate Names
class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein
Gene Symbol
POU5F1
UniProt
Additional OCT4 Products
Product Documents for OCT4 Antibody (OCT4/6875R)
Product Specific Notices for OCT4 Antibody (OCT4/6875R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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