OCT4 Antibody (OTI9B7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71225
![Western Blot: OCT4 Antibody (OTI9B7)Azide and BSA Free [NBP2-71225]](https://resources.bio-techne.com/images/products/OCT4-Antibody-OTI9B7-Azide-and-BSA-Free-Western-Blot-NBP2-71225-img0001.jpg "Western Blot: OCT4 Antibody (OTI9B7)Azide and BSA Free [NBP2-71225]")
![Western Blot: OCT4 Antibody (OTI9B7)Azide and BSA Free [NBP2-71225]](https://resources.bio-techne.com/images/products/OCT4-Antibody-OTI9B7-Azide-and-BSA-Free-Western-Blot-NBP2-71225-img0002.jpg "Western Blot: OCT4 Antibody (OTI9B7)Azide and BSA Free [NBP2-71225]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI9B7
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human POU5F1 (NP_002692) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
38.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for OCT4 Antibody (OTI9B7) - Azide and BSA Free
Western Blot: OCT4 Antibody (OTI9B7)Azide and BSA Free [NBP2-71225]
Western Blot: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4.![Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]](https://resources.bio-techne.com/images/products/OCT4-Antibody-OTI9B7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71225-img0003.jpg "Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]")
Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]
Immunocytochemistry/Immunofluorescence: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY 41186.![Immunohistochemistry: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]](https://resources.bio-techne.com/images/products/OCT4-Antibody-OTI9B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71225-img0005.jpg "Immunohistochemistry: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]")
Immunohistochemistry: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]
Immunohistochemistry: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225] - Staining of paraffin-embedded Human lung tissue using anti-41186 mouse monoclonal antibody.![Immunohistochemistry: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]](https://resources.bio-techne.com/images/products/OCT4-Antibody-OTI9B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71225-img0004.jpg "Immunohistochemistry: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]")
![Immunohistochemistry: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]](https://resources.bio-techne.com/images/products/OCT4-Antibody-OTI9B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71225-img0006.jpg "Immunohistochemistry: OCT4 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71225]")
Applications for OCT4 Antibody (OTI9B7) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Western Blot
1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: OCT4
Alternate Names
class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein
Gene Symbol
POU5F1
Additional OCT4 Products
Product Documents for OCT4 Antibody (OTI9B7) - Azide and BSA Free
Product Specific Notices for OCT4 Antibody (OTI9B7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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