OCT4 Antibody [DyLight 650]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2379C
![OCT4 Antibody [DyLight 650]](https://resources.bio-techne.com/images/products/nb100-2379c_rabbit-polyclonal-oct4-antibody-dylight-650-flow-intracellular--3092024155153..jpg "Detection of OCT4 in A431 Human Cell Line by Flow Cytometry.")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Primate
Predicted:
Feline (93%), Porcine (93%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
DyLight 650 (Excitation = 652 nm, Emission = 672 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Feline (93%), Porcine (93%). Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues. Bovine reactivity reported in scientific literature (PMID: 23054358).
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for OCT4 Antibody [DyLight 650]
Detection of OCT4 in A431 Human Cell Line by Flow Cytometry.
An intracellular stain was performed on A431 human skin carcinoma cell line using Rabbit anti-OCT4 Affinity Purified Polyclonal Antibody conjugated to DyLight 650 (Catalog # NB100-2379C, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.Applications for OCT4 Antibody [DyLight 650]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: OCT4
Alternate Names
class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein
Gene Symbol
POU5F1
Additional OCT4 Products
Product Documents for OCT4 Antibody [DyLight 650]
Product Specific Notices for OCT4 Antibody [DyLight 650]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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