OGG1 Antibody (2B4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52724
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0004.jpg "Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0005.jpg "Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
![Immunocytochemistry/ Immunofluorescence: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Immunocytochemistry-Immunofluorescence-NBP2-52724-img0002.jpg "Immunocytochemistry/ Immunofluorescence: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 2B4
Format
BSA Free
Concentration
0.9 mg/ml
Product Specifications
Immunogen
Partial recombinant protein made to an internal sequence of the human OGG1 protein (between amino acids 100-300)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for OGG1 Antibody (2B4) - BSA Free
Immunocytochemistry/ Immunofluorescence: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]
Immunocytochemistry/Immunofluorescence: OGG1 Antibody (2B4) [NBP2-52724] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (2B4) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0006.jpg "Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]
Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells renal cell carcinoma.![Flow (Intracellular): OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Flow-Intracellular-NBP2-52724-img0001.jpg "Flow (Intracellular): OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
Flow (Intracellular): OGG1 Antibody (2B4) - BSA Free [NBP2-52724]
Flow (Intracellular): OGG1 Antibody (2B4) [NBP2-52724] - An intracellular stain was performed on HeLa cells with OGG1 (2B4) antibody NBP2-52724 (Blue) and a matched isotype control NBP2-27231 (Orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).Applications for OGG1 Antibody (2B4) - BSA Free
Application
Recommended Usage
Flow Cytometry
2-5 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
0.9 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OGG1
Long Name
8-oxoGuanine DNA Glycosylase
Alternate Names
8-hydroxyguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, AP lyase, DNA-apurinic or apyrimidinic site lyase, HOGG1, MMH, MUTMOGH1HMMH, N-glycosylase/DNA lyase
Gene Symbol
OGG1
Additional OGG1 Products
Product Documents for OGG1 Antibody (2B4) - BSA Free
Product Specific Notices for OGG1 Antibody (2B4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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