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OIP5 Antibody (3G10-1D5)

Novus Biologicals, part of Bio-Techne | Catalog # H00011339-M01

Novus Biologicals, part of Bio-Techne
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H00011339-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3G10-1D5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

OIP5 (AAH15050, 1 a.a. ~ 229 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAQPLRHRSRCATPPRGDFCGGTERAIDQASFTTSMEWDTQVVKGSSPLGPAGLGAEEPAAGPQLPSWLQPERCAVFQCAQCHAVLADSVHLAWDLSRSLGAVVFSRVTNNVVLEAPFLVGIEGSLKGSTYNLLFCGSCGIPVGFHLYSTHAALAALRGHFCLSSDKMVCYLLKTKAIVNASEMDIQNVPLSEKIAELKEKIVLTHNRLKSLMKILSEVTPDQSKPEN

Reactivity Notes

Human. Other species not tested.

Specificity

OIP5 - Opa interacting protein 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for OIP5 Antibody (3G10-1D5)

Western Blot: OIP5 Antibody (3G10-1D5) [H00011339-M01]

Western Blot: OIP5 Antibody (3G10-1D5) [H00011339-M01]

Western Blot: OIP5 Antibody (3G10-1D5) [H00011339-M01] - Analysis of OIP5 expression in transfected 293T cell line by OIP5 monoclonal antibody (M01), clone 3G10-1D5.Lane 1: OIP5 transfected lysate(24.7 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: OIP5 Antibody (3G10-1D5) [H00011339-M01]

Immunoprecipitation: OIP5 Antibody (3G10-1D5) [H00011339-M01]

Immunoprecipitation: OIP5 Antibody (3G10-1D5) [H00011339-M01] - Analysis of OIP5 transfected lysate using anti-OIP5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OIP5 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: OIP5 Antibody (3G10-1D5) [H00011339-M01] - Detection limit for recombinant GST tagged OIP5 is 0.3 ng/ml as a capture antibody.

Applications for OIP5 Antibody (3G10-1D5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OIP5

Commonly referred to as OIP5, Opa interacting protein 5 is essential to protein binding. More specifically, OIP5 is necessary for the recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. OIP5 interacts with other proteins such as RAF1, BRAF, CENPA, CENPN, and HJURP and also binds UBC, TMPO, MIS18A, CASP10 and RARG.

Alternate Names

Cancer/testis antigen 86, CT865730547N13Rik, hMIS18beta, LAP2alpha interactor-25, LINT-25, MIS18B, MIS18beta, OIP-5, Opa interacting protein 5, Opa-interacting protein 5, protein Mis18-beta

Entrez Gene IDs

11339 (Human)

Gene Symbol

OIP5

OMIM

606020 (Human)

Additional OIP5 Products

Product Documents for OIP5 Antibody (3G10-1D5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OIP5 Antibody (3G10-1D5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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