Differentiated Rat Cortical Stem Cells (Catalog # NSC001) were stained with Mouse Anti-Human/Mouse/ Rat/Chicken Oligodendrocyte Marker O4 APC-conjugated Monoclonal Antibody (Catalog # FAB1326A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Oligodendrocyte Marker O4 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Differentiated Rat Cortical Stem Cells (Catalog # NSC001)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using FAB1326A in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oligodendrocyte Marker O4

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

References

Schachner, M. et al. (1981) Dev. Biol. 83:328.
Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
Gard, A. et al. (1995) Dev. Biol. 167:596.
Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
Cai, Z. et al. (2001) Brain Res. 898:126.

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Product Specific Notices for Oligodendrocyte Marker O4 APC-conjugated Antibody

For research use only

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Flow Cytometry

Oligodendrocyte Marker O4 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1326A

Key Product Details

Species Reactivity

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Applications

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Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Oligodendrocyte Marker O4 APC-conjugated Antibody

Detection of Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells by Flow Cytometry.