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OLLAS Epitope Tag Antibody (L2) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06713CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Epitope Tag

Applications

Chromatin Immunoprecipitation Sequencing, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG1 kappa Clone # L2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for OLLAS Epitope Tag Antibody (L2) [CoraFluor™ 1]

Application
Recommended Usage

Chromatin Immunoprecipitation Sequencing

Optimal dilutions of this antibody should be experimentally determined.

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry Whole-Mount

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: OLLAS Epitope Tag

The OLLAS (E.coli OmpF Linker and mouse Langerin fusion Sequence) epitope tag is a newly defined tag that is 100-fold more sensitive to other epitope tags tested, including Sigma's anti-FLAG(R) epitope tag antibody. OLLAS is a far superior tag for immunoprecipitation, fluorescent immunohistochemistry and flow cytometry detection.

Alternate Names

E.coli OmpF Linker and mouse Langerin fusion Sequence, OLLA-2

Additional OLLAS Epitope Tag Products

Product Documents for OLLAS Epitope Tag Antibody (L2) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OLLAS Epitope Tag Antibody (L2) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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