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OPA1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55290

Novus Biologicals, part of Bio-Techne
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NB110-55290
NB110-55290SS

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Chicken, Zebrafish

Cited:

Human, Mouse, Rat, Porcine, Fish - Danio rerio (Zebrafish)

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

A synthetic peptide made to an internal region within residues 500-600 of human OPA1.

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 23516612, 26365306).

Localization

Mitochondrion; mitochondrial inner membrane; single-pass membrane protein. Mitochondrion; mitochondrial intermembrane space.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

111 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OPA1 Antibody - BSA Free

Simple Western: OPA1 AntibodyBSA Free [NB110-55290]

Simple Western: OPA1 AntibodyBSA Free [NB110-55290]

Simple Western: OPA1 Antibody [NB110-55290] - Lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: OPA1 AntibodyBSA Free [NB110-55290]

Western Blot: OPA1 AntibodyBSA Free [NB110-55290]

Western Blot: OPA1 Antibody [NB110-55290] - Depletion of Oma1 in fish affects development. Immunoblot of protein extracts from injected fish (yolk removed) at 24, 48 and 72 hpf. Expression of Oma1, Opa1 isoforms (a-f) and Cox4 (mitochondrial abundance marker) was analyzed with respective antibodies. Actin and Ponceau S staining were loading controls. Source data (full-length blots) are available online in Supplementary information. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep13989), licensed under a CC-BY license.
Western Blot: OPA1 AntibodyBSA Free [NB110-55290]

Western Blot: OPA1 AntibodyBSA Free [NB110-55290]

Western Blot: OPA1 Antibody [NB110-55290] - Total protein from Human HeLa cells, Mouse MEF cells and Rat Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-OPA1 in 1% blocking buffer. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). The protein ladder was detected with an anti-mouse HRP secondary antibody and OPA1 with an anti-rabbit HRP secondary antibody using chemiluminescence.

Applications for OPA1 Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 25224038)

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 34923139)

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Simple Western

20 ug/ml

Western Blot

2 ug/ml
Application Notes
In Western blot, a band is seen at approx. 111 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB110-55290 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OPA1

OPA1 (optic atrophy-1) is a member of dynamin family of large GTPases and is a major player in fusion of the inner mitochondrial membrane and maintenance of cristae architecture, events which are orchestrated through oligomerization of differentially processed forms of OPA1. It exists as a single-pass membrane protein in the mitochondrion inner membrane as well as in soluble forms in mitochondrion intermembrane space, and is expressed in retina, brain, testis, heart, skeletal muscles etc. It binds with PARL and interacts with CHCHD3 as well as IMMT (preferentially with soluble OPA1 forms). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into mitochondrial intermembrane space. OPA1 form S1: inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion. Defects in OPA1 have been linked to optic atrophy type 1 (OPA1) and dominant optic atrophy plus syndrome (DOA+).

Long Name

Optic Atrophy Protein 1

Alternate Names

BERHS, EC 3.6.5.5, LargeG, lilr3, MGM1, MTDPS14, NPG, NTG

Gene Symbol

OPA1

Additional OPA1 Products

Product Documents for OPA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OPA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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