OPA1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-55290
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Chicken, Zebrafish
Cited:
Human, Mouse, Rat, Porcine, Fish - Danio rerio (Zebrafish)
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal region within residues 500-600 of human OPA1.
Reactivity Notes
Zebrafish reactivity reported in scientific literature (PMID: 23516612, 26365306).
Localization
Mitochondrion; mitochondrial inner membrane; single-pass membrane protein. Mitochondrion; mitochondrial intermembrane space.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
111 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for OPA1 Antibody - BSA Free
Simple Western: OPA1 AntibodyBSA Free [NB110-55290]
Simple Western: OPA1 Antibody [NB110-55290] - Lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Western Blot: OPA1 AntibodyBSA Free [NB110-55290]
Western Blot: OPA1 Antibody [NB110-55290] - Depletion of Oma1 in fish affects development. Immunoblot of protein extracts from injected fish (yolk removed) at 24, 48 and 72 hpf. Expression of Oma1, Opa1 isoforms (a-f) and Cox4 (mitochondrial abundance marker) was analyzed with respective antibodies. Actin and Ponceau S staining were loading controls. Source data (full-length blots) are available online in Supplementary information. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep13989), licensed under a CC-BY license.Western Blot: OPA1 AntibodyBSA Free [NB110-55290]
Western Blot: OPA1 Antibody [NB110-55290] - Total protein from Human HeLa cells, Mouse MEF cells and Rat Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-OPA1 in 1% blocking buffer. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). The protein ladder was detected with an anti-mouse HRP secondary antibody and OPA1 with an anti-rabbit HRP secondary antibody using chemiluminescence.Applications for OPA1 Antibody - BSA Free
Application
Recommended Usage
Immunoblotting
reported in scientific literature (PMID 25224038)
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 34923139)
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Simple Western
20 ug/ml
Western Blot
2 ug/ml
Application Notes
In Western blot, a band is seen at approx. 111 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using NB110-55290 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: OPA1
Long Name
Optic Atrophy Protein 1
Alternate Names
BERHS, EC 3.6.5.5, LargeG, lilr3, MGM1, MTDPS14, NPG, NTG
Gene Symbol
OPA1
Additional OPA1 Products
Product Documents for OPA1 Antibody - BSA Free
Product Specific Notices for OPA1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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